Fluorescence Plate Reader Black Plate

On the other hand black plates absorb light and reduce background and crosstalk.

Fluorescence plate reader black plate.

Top reading instruments rely on measuring reflected light above the wells. Find null cls3603 msds related peer reviewed papers technical documents similar products more at. Black or white clear bottomed low binding half area etc are some of the descriptions i came. The primary difference between white and black plates is their reflective properties.

The use of black plates will result in lower raw signals because the black color of the plate can quench the light. These are usually detection modes with a high signal yield and the use of a black microplate helps reducing background auto fluorescence and well to well crosstalk providing better signal blank ratios. Molecular probes 96 well microplates for fluorescence based assays are black walled clear bottom 96 well microplates that minimize well to well crosstalk and autofluorescence and so are optimized for fluorescence based applications. If microplate well size is interchangeable consider investing in a microplate reader that can accommodate a multitude of plate types such as a 6 to 384 well format.

If you have a top read option i would recommend that combined with a black plate. These plates are compatible with standard fluorescence microplate readers and are offered in a convenient 10. Consequently these plates are well suited for fluorescence intensity including fret and for fluorescence polarization assays. Thus white plates are commonly used for.

The use of white plates will result in higher raw signals because the light is reflected maximally by the white color of the plate. For luminescent assays white plates reflect light and will maximize light output signal. Alphaplate microplates are light gray plates designed to reduce cross talk while maintaining high signal in luminescent assays run in 384 well and 1536 well format. Which 96 well plate is recommended for alpha synuclein fluorescence in vitro assay with thioflavin t.

These plate provide higher signal to background and increased sensitivity compared to white or black plates in luminescent assays with cross talk similar to black plates. In my experience this is best for fluorescence eliminating cross talk and reducing background fluorescence. For fluorescent assays black plates absorb light and reduce background and crosstalk. Modern fluorescence microplate readers offer typically the option to read the fluorescence signal both from the top and from the bottom.

Instruments have become more compact to accommodate limited bench space without compromising reader intricacy or measurement speed. 96 multiwell plates 96 well microplates 96 well microtiter plates 96 well plates.